Flagellinolysin-DB: a database of flagellinolysin genes predicted from the NCBI

Last Updated: May 27, 2018

Summary: Flagellinolysin is a recently discovered variant of bacterial flagellin that contains a zinc-metalloproteinase within the central, hypervariable region. In Clostridium haemolyticum, flagellinolysin localizes to the surfaces of flagellar filaments and renders flagella with extracellular protease activity. The biological role of flagellum-mediated extracellular proteolysis is still unclear. Below is a list of all flagellinolysins that we have predicted from the NCBI to date.

References: Eckhard et al., Nat. Comm., 2017

Other Links: Flagellinolysin - MEROPS Peptidase Family 101

Contact: For more information, please contact Benjamin Jenkins (bjenkins [at] uwaterloo [dot] ca) or Andrew Doxey (acdoxey [at] uwaterloo [dot] ca)

Protein Fasta file of Predicted Flagellinolysins: flagellinolysins.fasta

Predicted flagellinolysins from the NCBI/genbank database
NCBI Accession Description Species
OFV71385.1 flagellin Acetobacterium wieringae
WP_084633525.1 hypothetical protein Acetobacterium wieringae
WP_014355249.1 flagellin Acetobacterium woodii
WP_007858923.1 MULTISPECIES: flagellin Acidovorax
WP_094287282.1 flagellin Acidovorax kalamii
WP_010460313.1 flagellin Acidovorax radicis
WP_099655197.1 flagellin Acidovorax sp. 56
WP_099740920.1 flagellin Acidovorax sp. 62
WP_063462804.1 flagellin Acidovorax sp. GW101-3H11
WP_108499185.1 flagellin Acidovorax sp. HMWF018
WP_108616954.1 flagellin Acidovorax sp. HMWF029
WP_057269442.1 flagellin Acidovorax sp. Root219
WP_057272374.1 flagellin Acidovorax sp. Root267
WP_057223076.1 flagellin Acidovorax sp. Root275
WP_056064769.1 flagellin Acidovorax sp. Root402
WP_056747048.1 flagellin Acidovorax sp. Root568
ODS66959.1 flagellin Acidovorax sp. SCN 65-108
WP_052211964.1 flagellin Anaerovibrio lipolyticus
WP_080325234.1 flagellin Anaerovibrio lipolyticus
WP_080325559.1 hypothetical protein Anaerovibrio lipolyticus
WP_081777614.1 hypothetical protein Anaerovibrio lipolyticus
WP_081777654.1 hypothetical protein Anaerovibrio lipolyticus
SHI55404.1 flagellin Anaerovibrio lipolyticus DSM 3074
ORT99876.1 Flagellin protein FlaA Anaerovibrio sp. JC8
WP_085023065.1 hypothetical protein Anaerovibrio sp. JC8
WP_085023690.1 hypothetical protein Anaerovibrio sp. JC8
WP_051533584.1 flagellin Anaerovibrio sp. RM50
WP_084276943.1 hypothetical protein Anaerovibrio sp. RM50
WP_084089987.1 flagellin Andreprevotia lacus
WP_040399502.1 flagellin Aquaspirillum serpens
WP_065340783.1 flagellin Azoarcus olearius
WP_011766260.1 flagellin Azoarcus sp. BH72
WP_035329181.1 flagellin Bacillus firmus
WP_083413185.1 hypothetical protein Bacillus mediterraneensis
WP_100332870.1 hypothetical protein Bacillus sp. FJAT-46582
WP_094768477.1 hypothetical protein Bacillus sp. OG2
WP_107901278.1 hypothetical protein Bacillus sp. OV186
WP_101631551.1 hypothetical protein Bacillus sp. V5-8f
SFK79987.1 flagellin Brevibacillus sp. OK042
OGA61394.1 flagellin Burkholderiales bacterium RIFCSPHIGHO2_01_FULL_64_960
OGB11850.1 flagellin Burkholderiales bacterium RIFCSPHIGHO2_02_FULL_64_19
WP_073390425.1 flagellin Butyrivibrio fibrisolvens
SCY53631.1 flagellin Butyrivibrio hungatei
SFQ43520.1 flagellin Butyrivibrio proteoclasticus
WP_026657293.1 flagellin Butyrivibrio sp. AC2005
WP_022772304.1 flagellin Butyrivibrio sp. AE2015
WP_081861040.1 hypothetical protein Butyrivibrio sp. AE2032
SCX88652.1 flagellin Butyrivibrio sp. INlla14
SDB60606.1 flagellin Butyrivibrio sp. INlla16
WP_081777783.1 hypothetical protein Butyrivibrio sp. LB2008
WP_026509870.1 flagellin Butyrivibrio sp. LC3010
WP_081676681.1 hypothetical protein Butyrivibrio sp. NC3005
SEG32478.1 flagellin C-terminal helical region Butyrivibrio sp. Su6
WP_081647772.1 hypothetical protein Butyrivibrio sp. VCB2006
WP_092325406.1 hypothetical protein Butyrivibrio sp. YAB3001
SEM50944.1 flagellin Butyrivibrio sp. ob235
PKK87909.1 hypothetical protein CVV64_21100 Candidatus Wallbacteria bacterium HGW-Wallbacteria-1
WP_047395168.1 flagellin Chitinibacter sp. ZOR0017
WP_028448441.1 flagellin Chitinibacter tainanensis
WP_027389515.1 flagellin Chrysiogenes arsenatis
OJV62414.1 hypothetical protein BGO41_06345 Clostridiales bacterium 38-18
KJF25817.1 hypothetical protein TZ02_16585 Clostridium aceticum
WP_082058301.1 hypothetical protein Clostridium aceticum
SHI59139.1 flagellin Clostridium amylolyticum
WP_039258875.1 flagellin Clostridium botulinum
BAB87738.1 flagellin protein FliA(H), partial Clostridium haemolyticum
WP_081964669.1 hypothetical protein Clostridium haemolyticum
KEI16196.1 hypothetical protein Z960_10360 Clostridium haemolyticum NCTC 9693
WP_080745757.1 hypothetical protein Clostridium novyi
WP_080965792.1 hypothetical protein Clostridium novyi
KGN03421.1 hypothetical protein Z969_00165 Clostridium novyi A str. 4570
KEH88343.1 hypothetical protein Z965_04930 Clostridium novyi A str. BKT29909
WP_079422951.1 flagellin Clostridium oryzae
WP_092475116.1 hypothetical protein Clostridium polysaccharolyticum
WP_092560591.1 hypothetical protein Clostridium populeti
WP_015394560.1 flagellin Clostridium saccharoperbutylacetonicum
WP_077365521.1 flagellin Clostridium saccharoperbutylacetonicum
WP_081897008.1 hypothetical protein Clostridium sp. KNHs214
WP_085298121.1 hypothetical protein Colwellia mytili
WP_019027008.1 flagellin Colwellia piezophila
WP_101233471.1 hypothetical protein Colwellia sp. 12G3
WP_101230504.1 hypothetical protein Colwellia sp. 75C3
WP_070374637.1 flagellin Colwellia sp. PAMC 20917
WP_043380247.1 flagellin Comamonas aquatica
WP_066532860.1 flagellin Comamonas terrigena
WP_015263259.1 flagellin/flagellar hook associated protein Desulfitobacterium dichloroeliminans
WP_035245515.1 flagellin Desulfobulbus mediterraneus
WP_081468591.1 hypothetical protein Desulfosporosinus orientis
AET69168.1 flagellin/flagellar hook associated protein Desulfosporosinus orientis DSM 765
WP_006000355.1 flagellin-like Desulfuromonas acetoxidans
WP_085544068.1 hypothetical protein Dethiosulfovibrio salsuginis
OLN24035.1 hypothetical protein BTO30_01045 Domibacillus antri
WP_083632250.1 hypothetical protein Domibacillus antri
EOS80891.1 hypothetical protein C817_01227 Dorea sp. 5-2
WP_022745605.1 flagellin Dorea sp. 5-2
WP_004602238.1 flagellin/flagellar hook associated protein Eubacterium cellulosolvens
WP_035385003.1 flagellin Ferrimonas futtsuensis
WP_084144490.1 flagellin Ferrimonas kyonanensis
SDI85333.1 flagellin Ferrimonas sediminum
WP_090363492.1 hypothetical protein Ferrimonas sediminum
WP_084478163.1 hypothetical protein Fervidicella metallireducens
EYE88791.1 flagellin Fervidicella metallireducens AeB
PKM60925.1 hypothetical protein CVU99_05685 Firmicutes bacterium HGW-Firmicutes-4
PKM48348.1 hypothetical protein CVV01_03965 Firmicutes bacterium HGW-Firmicutes-6
WP_091193990.1 hypothetical protein Formivibrio citricus
PCH97338.1 flagellin Gammaproteobacteria bacterium
WP_108652164.1 hypothetical protein Gammaproteobacteria bacterium DM2
WP_108652165.1 hypothetical protein Gammaproteobacteria bacterium DM2
PKM23960.1 hypothetical protein CVV09_19000 Gammaproteobacteria bacterium HGW-Gammaproteobacteria-13
OIQ47118.1 hypothetical protein BM565_08285 Gammaproteobacteria bacterium MedPE
WP_088533867.1 flagellin Geobacter sp. DSM 9736
WP_006299301.1 flagellin Hylemonella gracilis
WP_035605832.1 flagellin Hylemonella gracilis
WP_102401946.1 hypothetical protein Khelaifiella massiliensis
SKB95611.1 flagellin Lachnospiraceae bacterium
WP_079485607.1 flagellin Lachnospiraceae bacterium
SFL49366.1 flagellin Lachnospiraceae bacterium KH1T2
WP_089973523.1 hypothetical protein Lachnospiraceae bacterium KH1T2
EOS35535.1 hypothetical protein C808_04738 Lachnospiraceae bacterium M18-1
WP_081643830.1 hypothetical protein Lachnospiraceae bacterium M18-1
WP_044921500.1 flagellin Lachnospiraceae bacterium MA2020
WP_049973154.1 flagellin Lachnospiraceae bacterium MC2017
WP_081834044.1 hypothetical protein Lachnospiraceae bacterium MC2017
WP_051665872.1 flagellin Lachnospiraceae bacterium ND2006
SCY50476.1 flagellin C-terminal helical region Lachnospiraceae bacterium XBB2008
WP_084591276.1 hypothetical protein Lysinibacillus sinduriensis
KGR76499.1 hypothetical protein CD33_06415 Lysinibacillus sinduriensis BLB-1 = JCM 15800
WP_106785126.1 hypothetical protein Lysinibacillus sp. Marseille-P5727
SOB91075.1 flagellin Lysinibacillus xyleni
WP_097072346.1 hypothetical protein Lysinibacillus xyleni
WP_084403915.1 flagellin Marinobacterium litorale
WP_056129008.1 MULTISPECIES: flagellin Massilia
WP_084416490.1 flagellin Massilia alkalitolerans
WP_020655191.1 flagellin Massilia niastensis
WP_105379546.1 flagellin Massilia phosphatilytica
WP_075795119.1 flagellin Massilia putida
WP_036238512.1 flagellin Massilia sp. JS1662
WP_078032628.1 flagellin Massilia sp. KIM
WP_091659287.1 hypothetical protein Massilia sp. PDC64
WP_056440654.1 flagellin Massilia sp. Root335
WP_083223894.1 flagellin Methyloversatilis sp. RAC08
WP_018410044.1 flagellin Methyloversatilis thermotolerans
WP_090696930.1 flagellin Nitrosomonas aestuarii
WP_107795035.1 flagellin Nitrosomonas aestuarii
WP_090628462.1 hypothetical protein Nitrosomonas marina
WP_090659134.1 flagellin Nitrosomonas marina
WP_090320298.1 hypothetical protein Nitrosomonas oligotropha
WP_107801550.1 flagellin Nitrosomonas oligotropha
WP_108698042.1 flagellin Nitrosomonas sp. Nm141
WP_090333704.1 flagellin Nitrosomonas sp. Nm51
WP_085756847.1 hypothetical protein Oceanicoccus sagamiensis
WP_101757483.1 flagellin Oceanicoccus sp. KOV_DT_Chl
SET07793.1 flagellin Oceanobacillus limi
WP_090868566.1 hypothetical protein Oceanobacillus limi
WP_078320399.1 MULTISPECIES: flagellin Oceanospirillales
WP_028300311.1 flagellin Oceanospirillum beijerinckii
OOV86287.1 hypothetical protein BTA35_0213785 Oceanospirillum linum
WP_028305148.1 flagellin Oceanospirillum maris
WP_078743693.1 flagellin Oceanospirillum multiglobuliferum
WP_086480435.1 hypothetical protein Oceanospirillum sanctuarii
SEG46837.1 flagellin Oleiphilus messinensis
KGE20414.1 hypothetical protein PWYN_14485 Paenibacillus wynnii
WP_084146751.1 hypothetical protein Paenibacillus wynnii
WP_069313537.1 flagellin Piscirickettsia litoralis
OQW40921.1 hypothetical protein A4S08_03460 Proteobacteria bacterium SG_bin4
OQW83514.1 hypothetical protein BVN30_05905 Proteobacteria bacterium ST_bin16
WP_091981617.1 hypothetical protein Pseudoalteromonas denitrificans
SFC23839.1 flagellin Pseudoalteromonas denitrificans DSM 6059
PAJ71655.1 hypothetical protein CJF42_25555 Pseudoalteromonas sp. NBT06-2
WP_095365893.1 hypothetical protein Pseudoalteromonas sp. NBT06-2
WP_095369312.1 hypothetical protein Pseudoalteromonas sp. NBT06-2
WP_083781262.1 hypothetical protein Pseudoalteromonas tunicata
EAR29544.1 flagellar protein Pseudoalteromonas tunicata D2
WP_086743873.1 hypothetical protein Pseudoalteromonas ulvae
WP_090166731.1 MULTISPECIES: hypothetical protein Pseudobutyrivibrio
SEK40633.1 flagellin Pseudobutyrivibrio ruminis
WP_033152383.1 flagellin Pseudobutyrivibrio ruminis
WP_083380635.1 hypothetical protein Pseudobutyrivibrio ruminis
WP_097077247.1 hypothetical protein Pseudobutyrivibrio ruminis
SOC17135.1 flagellin Pseudobutyrivibrio ruminis DSM 9787
SDH28935.1 flagellin Pseudobutyrivibrio sp. 49
SEA22865.1 flagellin Pseudobutyrivibrio sp. ACV-2
WP_090298398.1 hypothetical protein Pseudobutyrivibrio sp. ACV-2
WP_090395246.1 hypothetical protein Pseudobutyrivibrio sp. C4
WP_044939094.1 flagellin Pseudobutyrivibrio sp. LB2011
WP_028235328.1 flagellin Pseudobutyrivibrio sp. MD2005
SFN52660.1 flagellin Pseudobutyrivibrio sp. UC1225
OHC21989.1 hypothetical protein A3J71_13410 Pseudomonadales bacterium RIFCSPHIGHO2_02_FULL_60_43
WP_108486670.1 MULTISPECIES: flagellin Pseudomonas
WP_021220822.1 flagellin Pseudomonas alcaligenes
WP_043240123.1 flagellin Pseudomonas alcaligenes
SDP97597.1 flagellin Pseudomonas guguanensis
WP_090432186.1 hypothetical protein Pseudomonas guguanensis
WP_071872944.1 flagellin Pseudomonas hussainii
WP_074869101.1 flagellin Pseudomonas hussainii
WP_080757018.1 hypothetical protein Pseudomonas mendocina
WP_037049563.1 flagellin Pseudomonas oleovorans
WP_083422385.1 flagellin Pseudomonas otitidis
WP_090248607.1 hypothetical protein Pseudomonas peli
WP_107329422.1 hypothetical protein Pseudomonas pseudoalcaligenes
PJE43654.1 hypothetical protein CUR33_07205 Pseudomonas sp.
OEC41128.1 hypothetical protein A7D27_14485 Pseudomonas sp. 1D4
WP_083265639.1 flagellin Pseudomonas sp. 1D4
WP_082805330.1 flagellin Pseudomonas sp. BMS12
OEC61717.1 hypothetical protein A9G05_01600 Pseudomonas sp. ENNP23
WP_083272481.1 flagellin Pseudomonas sp. ENNP23
KIV71406.1 Flagellin protein FlaB Pseudomonas sp. FeS53a
WP_044405966.1 flagellin Pseudomonas sp. FeS53a
WP_043308674.1 flagellin Pseudomonas sp. ML96
OYT95880.1 flagellin Pseudomonas sp. PGPPP3
WP_108105530.1 flagellin Pseudomonas sp. TC-11
SEI78234.1 flagellin Pseudospirillum japonicum
WP_093310986.1 hypothetical protein Pseudospirillum japonicum
WP_084605300.1 hypothetical protein Psychromonas aquimarina
WP_083938418.1 hypothetical protein Psychromonas ossibalaenae
WP_101107062.1 hypothetical protein Psychromonas sp. psych-6C06
WP_105052320.1 hypothetical protein Psychrosphaera saromensis
WP_054620994.1 flagellin Rhodocyclaceae bacterium Paddy-1
WP_094756614.1 hypothetical protein Schwartzia succinivorans
SHF25371.1 flagellin Schwartzia succinivorans DSM 10502
PLY16733.1 hypothetical protein C0631_02985 Sedimenticola sp.
WP_081626335.1 hypothetical protein Selenomonas bovis
SDP09567.1 flagellin Selenomonas ruminantium
SEA22530.1 flagellin Selenomonas ruminantium
SEH29561.1 flagellin Selenomonas ruminantium
SEH29565.1 Flagellin FlgL Selenomonas ruminantium
SFB11500.1 flagellin Selenomonas ruminantium
SFI13140.1 flagellin Selenomonas ruminantium
SFT90017.1 flagellin Selenomonas ruminantium
SFW31537.1 flagellin Selenomonas ruminantium
WP_072305930.1 flagellin Selenomonas ruminantium
WP_074672950.1 flagellin Selenomonas ruminantium
WP_074697577.1 flagellin Selenomonas ruminantium
WP_075428929.1 flagellin Selenomonas ruminantium
WP_080657033.1 hypothetical protein Selenomonas ruminantium
WP_080695352.1 flagellin Selenomonas ruminantium
WP_080695464.1 hypothetical protein Selenomonas ruminantium
WP_080705950.1 hypothetical protein Selenomonas ruminantium
WP_081342451.1 hypothetical protein Selenomonas ruminantium
WP_081342479.1 flagellin Selenomonas ruminantium
WP_081348474.1 hypothetical protein Selenomonas ruminantium
WP_081351405.1 hypothetical protein Selenomonas ruminantium
WP_081368530.1 hypothetical protein Selenomonas ruminantium
WP_081371917.1 flagellin Selenomonas ruminantium
WP_082334500.1 hypothetical protein Selenomonas ruminantium
WP_082336258.1 hypothetical protein Selenomonas ruminantium
WP_102012100.1 hypothetical protein Selenomonas ruminantium
WP_102013454.1 hypothetical protein Selenomonas ruminantium
WP_102013490.1 hypothetical protein Selenomonas ruminantium
BAL82576.1 putative flagellin Selenomonas ruminantium subsp. lactilytica TAM6421
BAL84184.1 putative flagellin Selenomonas ruminantium subsp. lactilytica TAM6421
WP_028129564.1 hypothetical protein Selenomonas sp. AE3005
WP_081824787.1 hypothetical protein Selenomonas sp. FC4001
WP_078484527.1 flagellin Solemya velesiana gill symbiont
WP_094606130.1 hypothetical protein Sporomusa silvacetica
WP_044831744.1 flagellin Thalassomonas actiniarum
WP_044838529.1 flagellin Thalassomonas viridans
SDY61893.1 flagellin Tindallia californiensis
WP_093312285.1 hypothetical protein Tindallia californiensis
OUU11153.1 hypothetical protein CBC00_04115 Verrucomicrobia bacterium TMED40
OUU27733.1 hypothetical protein CBC04_02650 Verrucomicrobia bacterium TMED44
OUU29002.1 hypothetical protein CBC04_00430 Verrucomicrobia bacterium TMED44
OUU29003.1 hypothetical protein CBC04_00435 Verrucomicrobia bacterium TMED44
OUU43446.1 hypothetical protein CBC16_02770 Verrucomicrobia bacterium TMED56
WP_047967967.1 flagellin Vogesella sp. EB

Note: HTML table above was generated using the following command:

Instructions for generating this dataset

  1. PSI-BLAST (two iterations) with the following query

  2. Download PSI-BLAST hits (full sequences) as fasta file
  3. Run hmm-scan on fasta file
  4. Copy and paste results table into hmmresults.txt and do the following
  5. cat hmmresults.txt | grep "Flagellin" | awk '{print $2}'
  6. Go here to retrieve FASTA file - save as flagellinolysins.fasta

Copyright © 2018, Andrew Doxey, University of Waterloo